-
Guide RNA (g
RNA) or
single guide RNA (
sgRNA) is a
short sequence of
RNA that
functions as a
guide for the Cas9-endonuclease or
other Cas-proteins that...
- CRISPR-Cas9 gene
editing system,
coupled with
libraries of
single guide RNAs (
sgRNAs),
which are
designed to
target every gene in the genome. Over recent...
-
Depletion or
enrichment of
sgRNAs is
detected and
compared to the
original sgRNA library,
annotated with the
target gene that
sgRNA corresponds to. Statistical...
- the
RNA used for
targeting Cas9 to a
genomic location.
Since sgRNAs are
usually expressed by
RNA polymerase III,
which limits the
length of the
RNA domain...
- or
whole proteins linked to dCas9 or
sgRNAs that ****ist in the
recruitment of
important co-factors as well as
RNA Polymerase for
transcription of the gene(s)...
- many
sgRNAs simultaneously is to
array the
sgRNAs in a
single construct with
multiple promoters or
processing elements. For example, Extra-Long
sgRNA Arrays...
- cr
RNA-tracr
RNA complex is
replaced by a
chimeric single-guide
RNA (
sgRNA, in red)
which has been
proved to have the same
function as the
natural RNA complex...
-
Hegde M,
Smith I, et al. (December 2014). "Rational
design of
highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation".
Nature Biotechnology. 32 (12):...
- Cas12a's
small cr
RNAs are
ideal for
multiplexed genome editing, as more of them can be
packaged in one
vector than can Cas9's
sgRNAs. The
sticky 5′ overhangs...
- MK,
Giraldez AJ (October 2015). "CRISPRscan:
designing highly efficient sgRNAs for CRISPR-Cas9
targeting in vivo".
Nature Methods. 12 (10): 982–8. doi:10...
