-
Dideoxynucleotides are chain-elongating
inhibitors of DNA polymerase, used in the
Sanger method for DNA sequencing. They are also
known as 2',3' because...
- electrop****sis and is
based on the
random incorporation of chain-terminating
dideoxynucleotides by DNA
polymerase during in
vitro DNA replication.
After first being...
- (LNA),
morpholinos and
peptide nucleic acids (PNA). In sequencing,
dideoxynucleotides are used.
These nucleotides possess the non-canonical
sugar dideoxyribose...
- labeled-primer approach. This is
accomplished by
labelling each of the
dideoxynucleotide chain-terminators with a
separate fluorescent dye,
which fluoresces...
- "Structure-based
design of Taq DNA
polymerases with
improved properties of
dideoxynucleotide incorporation".
Proceedings of the
National Academy of
Sciences of...
-
Hybridization is
followed by single-base
extension with hapten-labeled
dideoxynucleotides. The
ddCTP and
ddGTP are
labeled with
biotin while ddATP and ddUTP...
-
which is
based on the
selective incorporation of chain-terminating
dideoxynucleotides by DNA
polymerase during in
vitro DNA replication. Recently, shotgun...
-
which used a
novel fluorescent labeling technique enabling all four
dideoxynucleotides to be
identified in a
single lane. By 1990, the U.S.
National Institutes...
-
polymerase primed synthesis ("plus and minus")
technique and, eventually,
dideoxynucleotide chain-terminating sequencing,
which was
later termed Sanger sequencing...
- or, for PNA,
other methods of
peptide synthesis.[citation needed]
Dideoxynucleotides are used in sequencing.
These nucleoside triphosphates possess a non-canonical...
