-
Avidin is a
tetrameric biotin-binding
protein produced in the
oviducts of birds,
reptiles and
amphibians and
deposited in the
whites of
their eggs. Dimeric...
-
Neutralite Avidin protein is a
deglycosylated version of
chicken avidin, with a m**** of
approximately 60,000 daltons. As a
result of
carbohydrate removal...
- the
advantage of much
lower nonspecific binding than
avidin.
Deglycosylated avidin (Neutr
Avidin) is more
comparable to the size, pI, and
nonspecific binding...
-
incubated with
avidin bound to beads, then rinsed,
removing all
unbound proteins,
while leaving only the
biotinylated protein bound to
avidin. Last, the biotinylated...
-
small size of
biotin (MW = 244.31 g/mol).
Biotin binds to
streptavidin and
avidin with an
extremely high affinity, fast on-rate, and high specificity, and...
-
several months may
result in
biotin deficiency, due to
their avidin content, as the
avidin tightly binds biotin and
prevents its absorption. Egg white...
- and enzymes, as well as rRNA and dsDNA.
Avidin biotin media is used in the
purification process of biotin/
avidin and
their derivatives.
Carbohydrate bonding...
- chromatography,
affinity labeling,
affinity therapy, and the
avidin-biotin system. The
avidin-biotin
complex is the
highest affinity interaction in nature...
-
different methods for
attaining a
higher fluorop****-antigen
ratio such as the
Avidin-Biotin
Complex (ABC method) and
Labeled Streptavidin-Biotin (LSAB method)...
- Wilchek, M.; Sussman, J. (1993). "Three-dimensional
structures of
avidin and the
avidin-biotin complex".
Proceedings of the
National Academy of Sciences...
