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Streptavidin /ˌstrɛpˈtævɪdɪn/ is a 52 kDa
protein (tetramer)
purified from the
bacterium Streptomyces avidinii.
Streptavidin homo-tetramers have an extraordinarily...
- due to the
small size of
biotin (MW = 244.31 g/mol).
Biotin binds to
streptavidin and
avidin with an
extremely high affinity, fast on-rate, and high specificity...
- with
excess free biotin. The
process can also
utilize bacteria-derived
streptavidin bound to beads, but
because it has a
higher dissociation constant than...
- by
binding biotin helpful for
bacterial growth. As
evidence for this,
streptavidin, a
related protein with
equal biotin affinity and a very
similar binding...
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Streptavidin is a
tetrameric protein expressed in
Streptomyces avidinii.
Because of
Streptavidin's high
affinity for
vitamin H (biotin),
Streptavidin...
-
cytokine was not
washed away when the
wells were rinsed.
Streptavidin-enzyme conjugate:
Streptavidin-enzyme
conjugate is
added to the
wells in
order to bind...
-
resolution microscopy Strep-tag, a
peptide which binds to
streptavidin or the
modified streptavidin called streptactin (Strep-tag II: WSHPQFEK) T7-tag, an...
- fluorescently-labelled
streptavidin or antibodies, respectively. CISH is
performed using indirect labelling in
which antibodies or
streptavidin are conjugated...
- cases, the ****ociations can be
quite strong—for example, the
protein streptavidin and the
vitamin biotin have a
dissociation constant (reflecting the ratio...
- fluorop****-antigen
ratio such as the Avidin-Biotin
Complex (ABC method) and
Labeled Streptavidin-Biotin (LSAB method).
Immunofluorescence is only
limited to
fixed (i...
