- In
materials science and
molecular biology,
thermostability is the
ability of a
substance to
resist irreversible change in its
chemical or
physical structure...
- Taq
polymerase is a
thermostable DNA
polymerase I
named after the
thermophilic eubacterial microorganism Thermus aquaticus, from
which it was originally...
- the high
temperature (96 °C) step
required to
denature DNA. In 2013, a
thermostable group II
intron reverse transcriptase (TGIRT), GsI-IIC-MRF, from G. stearothermophilus...
-
their applications in DNA
replication techniques, such as PCR,
where thermostable enzymes are
necessary for
proper DNA replication.
Enzyme function at...
-
temperature of 94–96 °C (201–205 °F), or 98 °C (208 °F) if
extremely thermostable polymerases are used,
which is then held for 1–10 minutes. Denaturation:...
-
motility or
remain immotile. T.
aquaticus has
become famous as a
source of
thermostable enzymes,
particularly the Taq DNA polymerase, as
described below. Studies...
-
Protein tertiary structure is the three-dimensional
shape of a protein. The
tertiary structure will have a
single polypeptide chain "backbone" with one...
-
enzymes require a
metal ion co-factor.
Thermostable proteins are
often more
useful than
their non-
thermostable counterparts, e.g., DNA
polymerase in the...
- the
polymerase chain reaction (PCR) process,
before being replaced by
thermostable enzymes such as Taq polymerase. Just as the 5' → 3'
exonuclease activity...
-
thermal cycles (94 °C/80 °C) or 16
hours of cycling.10 This
exceptional thermostability permits extremely high
hybridization stringency and
ligation specificity...
