-
molecular biology,
subcloning is a
technique used to move a
particular DNA
sequence from a
parent vector to a
destination vector.
Subcloning is not to be confused...
- T cloning) is a
subcloning technique that
avoids the use of
restriction enzymes and is
easier and
quicker than
traditional subcloning. The
technique relies...
- strand. The
concept is used in
molecular biology, in cloning, or when
subcloning insert DNA into
vector DNA. Such ends may be
generated by restriction...
- electrop****sis, and chromatography. It is used in
genetic fingerprinting,
plasmid subcloning, and RFLP analysis. A
given restriction enzyme cuts DNA
segments within...
-
reported in 1973. A neuroblast-like
subclone of SK-N-SH,
named SH-SY, was
subcloned as SH-SY5,
which was
subcloned a
third time to
produce the SH-SY5Y...
- genomes.
Cancers usually show
tumour heterogeneity,
containing multiple subclones. They also
frequently have
reduced expression of DNA
repair enzymes due...
- One
application for this
practice is for use in TA cloning, a
special subcloning technique similar to PCR,
where efficiency can be
increased by adding...
- 2011.07.023. PMC 3169815. PMID 21855840. Roach, J. C. (1995). "Random
subcloning".
Genome Research. 5 (5): 464–73. doi:10.1101/gr.5.5.464. PMID 8808467...
- DNA
fragment has been
cloned into a
cloning vector, it may be
further subcloned into
another vector designed for more
specific use.
There are many types...
- "Double-Stranded RNA
Activated Caspase Oligomerizer (DRACO): Design,
Subcloning, and
Antiviral Investigation".
Journal of
Applied Biotechnology Reports...
