-
molecular biology,
subcloning is a
technique used to move a
particular DNA
sequence from a
parent vector to a
destination vector.
Subcloning is not to be confused...
- T cloning) is a
subcloning technique that
avoids the use of
restriction enzymes and is
easier and
quicker than
traditional subcloning. The
technique relies...
- strand. The
concept is used in
molecular biology, in cloning, or when
subcloning insert DNA into
vector DNA. Such ends may be
generated by restriction...
- electrop****sis, and chromatography. It is used in
genetic fingerprinting,
plasmid subcloning, and RFLP analysis. A
given restriction enzyme cuts DNA
segments within...
-
reported in 1973. A neuroblast-like
subclone of SK-N-SH,
named SH-SY, was
subcloned as SH-SY5,
which was
subcloned a
third time to
produce the SH-SY5Y...
- genomes.
Cancers usually show
tumour heterogeneity,
containing multiple subclones. They also
frequently have
reduced expression of DNA
repair enzymes due...
- may give rise to
subclones that
possess an
evolutionary advantage over the
others within the
tumour environment, and
these subclones may
become dominant...
- One
application for this
practice is for use in TA cloning, a
special subcloning technique similar to PCR,
where efficiency can be
increased by adding...
-
Cloning vectors today are
essential not only for
routine gene
isolation and
subcloning, but also as the
basis for
advanced molecular biology tools. They are...
- "Double-Stranded RNA
Activated Caspase Oligomerizer (DRACO): Design,
Subcloning, and
Antiviral Investigation".
Journal of
Applied Biotechnology Reports...
