-
principle any gene can be
knocked down by a
synthetic siRNA with a
complementary sequence,
siRNAs are an
important tool for
validating gene
function and...
- long double-stranded
RNA (ds
RNA)
molecules into
short double-stranded
fragments of
approximately 21 to 23
nucleotide siRNAs. Each
siRNA is
unwound into two...
- sequence-specific
cleavage of
target m
RNA. Ta-
siRNAs are
classified as
siRNA because they
arise from double-stranded
RNA (ds
RNA). ta-
siRNA were
originally detected...
-
triggers m
RNA degradation by 5' decapping, 3' poly(A) tail removal, or
endonucleolytic cleavage. In metazoans,
small interfering RNAs (
siRNAs) processed...
-
resemble the
small interfering RNAs (
siRNAs) of the
RNA interference (RNAi) pathway,
except mi
RNAs derive from
regions of
RNA transcripts that fold back on themselves...
- of
small RNA have
currently been identified, namely:
small interfering RNA (
siRNA), micro
RNA (mi
RNA), and piwi-interacting
RNA (pi
RNA).
siRNAs act in the...
- RNA-dependent
RNA polymerase (RdRp) to
generate more
siRNAs. In
Schizosaccharomyces pombe and Arabidopsis, the
processing of ds
RNA targets into
siRNA by Dicer...
- nc
RNAs from
small non-coding
RNAs, such as micro
RNAs (mi
RNAs),
small interfering RNAs (
siRNAs), Piwi-interacting
RNAs (pi
RNAs),
small nucleolar RNAs (snoRNAs)...
- has been
found that
exogeneous siRNAs only last a few days (a few w****s at most in non-dividing cells) in vivo. If
siRNA is able to
successfully reach...
-
transcribed m
RNAs (IVT-m
RNA) have been used to
deliver proteins for bone regeneration, pluripotency, and
heart function in
animal models.
SiRNAs,
short RNA molecules...
