- In spectroscopy, an
isosbestic point is a
specific wavelength,
wavenumber or
frequency at
which the
total absorbance of a
sample does not
change during...
- maxima) for the
individual species. None of the
wavelengths may be an
isosbestic point for a pair of species. The set of the
following simultaneous equations...
- 4-nitrophenol has a weak
absorbance at 405 nm (ε = 0.2 mM−1 cm−1). The
isosbestic point for 4-nitrophenol/4-nitrophenoxide is at 348 nm, with ε = 5.4 mM−1...
-
characteristic blue-green color. The acid and
basic forms of this dye have an
isosbestic point in
their UV-Visible spectrum,
around 515 nm,
indicate that the two...
-
densities of a
blood sample flowing through small-bore gl****
tubes at
isosbestic wavelengths for
deoxyhemoglobin and
oxyhemoglobin and the
product of the...
-
shifts in
neutral pH.
Spectroscopic superposition indicated TNP-ATP’s
isosbestic point to be 339 nm. At low
concentrations of TNP-ATP (≤1 μM), fluorescent...
-
source of
linearly polarized light with a
wavelength of 550 nanometers, an
isosbestic point for hemoglobin, thus
imaging the
erythrocytes as they are flowing...
- or to
identify compounds. Both are
forms of
vibrational spectroscopy.
Isosbestic point – a
wavelength where absorption does not
change as the reaction...
-
formed in situ to give oxidized, ring-opened products.
Fluorescein has an
isosbestic point (equal
absorption for all pH values) at 460 nm. Many derivatives...
- its
isosbestic signal are
approximately 470 nm and 415 nm (blue), respectively. The goal is to photo-excite the
maximum absorption and
isosbestic points...
