-
reads 5'-
GAATTC-3',
while the
bottom strand reads 3'-CTTAAG-5'. If the DNA
strand is
flipped over, the
sequences are
exactly the same (5'
GAATTC-3' and 3'-CTTAAG-5')...
- overhangs. For example, the
nuclease EcoRI has the
recognition sequence 5'—
GAATTC—3'. When the
enzyme encounters this sequence, it
cleaves each
backbone between...
- the
common restriction enzyme EcoRI recognizes the
palindromic sequence GAATTC and cuts
between the G and the A on both the top and
bottom strands. This...
-
Enzyme Source Recognition Sequence Cut
EcoRI Escherichia coli 5'
GAATTC 3'CTTAAG 5'---G AATTC---3' 3'---CTTAA G---5'
EcoRII Escherichia coli 5'CCWGG 3'GGWCC...
- For instance, the
EcoRI enzyme recognizes and
cleaves the
sequence 5' –
GAATTC – 3'.
Restriction endonucleases come in
several types. A
restriction endonuclease...
- Csp68KI, DsaIV, Eco47I, SinI
AflII 39
Anabaena flos-aquae 5'
CTTAAG 3'
GAATTC 5' ---C TTAAG--- 3' 3' ---GAATT C--- 5' BfrI, BspTI, Bst98I, BstAFI...
- EcoT22I, Mph1103I, NsiI, SepI, SspD5II Ppu111I
Pseudomonas putida P111 5'
GAATTC 3'
CTTAAG 5' ---G AATTC--- 3' 3' ---CTTAA G--- 5'
PpuAI Pseudomonas...
- BspT107I, Eco64I, HgiCI, HgiHI,
PfaAI MspCI Micrococcus sp. 5'
CTTAAG 3'
GAATTC 5' ---C TTAAG--- 3' 3' ---GAATT C--- 5' MspR9I
Micrococcus sp. R9 5'...
- 5'-GAAN7RTCG-3' 5'
EcoRI 1QC9,
Search EcoR1
structures Escherichia coli RY13 5'
GAATTC 3'
CTTAAG 5' ---G AATTC--- 3' 3' ---CTTAA G--- 5'
EcoRII 1NA6 Escherichia...
- ---****N5RTCN8NNNNNN --- 3' 3' ---CTRN5YAGN8N NNNNN--- 5' HalI
Hafnia alvei B6 5'
GAATTC 3'
CTTAAG 5' ---G AATTC--- 3' 3' ---CTTAA G--- 5'
HalII Hafnia alvei...
