- A
relaxase is a single-strand DNA
transesterase enzyme produced by some
prokaryotes and viruses.
Relaxases are
responsible for site- and strand-specific...
-
initiated by a
signal the
relaxase enzyme creates a nick in one of the
strands of the
conjugative plasmid at the oriT.
Relaxase may work
alone or in a complex...
- The traA gene
codes for
relaxase,
which is an
enzyme that
initiates plasmid DNA
transfer during bacterial conjugation.
Relaxase forms a
relaxosome complex...
- tend to be smaller, on the
order of 10-20 kDa
while nicking domains from
relaxases are larger,
roughly 20-40 kDa in size. HUH
endonucleases generally have...
-
initiated by a
signal the
relaxase enzyme creates a nick in one of the
strands of the
conjugative plasmid at the oriT.
Relaxase may work
alone or in a complex...
- the
origin of transfer, oriT. The most
important of
these proteins is
relaxase,
which is
responsible for
beginning the
conjugation process by cutting...
- the oriT is both an
enzymatic substrate and
recognition site for the
relaxase proteins.
Relaxosomes have oriT-specific
auxiliary factors that help it...
- VirD2
relaxase is
aided by the
accessory factors VirD1, VirC1 and VirC2
while it
processes the DNA substrate. Furthermore, the VirD2
relaxase and the...
-
strand of DNA,
called the T-strand, is cut at nic by an
enzyme called relaxase. This
single strand is
eventually transferred to the
recipient cell during...
- made by a replicon-encoded endonuclease,
variously called the nickase,
relaxase,
mobilization protein (mob), transesterase, or
replication protein (Rep)...
