- In
genetic engineering, a gene gun or
biolistic particle delivery system is a
device used to
deliver exogenous DNA (transgenes), RNA, or
protein to cells...
- in
genetic engineering: The
plasmid method, the
vector method and the
biolistic method. This
technique is used
mainly for
microorganisms such as bacteria...
- membrane-repair mechanisms.
Another method used to
transform plant cells is
biolistics,
where particles of gold or
tungsten are
coated with DNA and then shot...
-
insertion of
genetic material into
plant cells.
Other methods include biolistics,
where particles of gold or
tungsten are
coated with DNA and then shot...
- the DNA is
often inserted using Agrobacterium-mediated recombination,
biolistics or electroporation. As only a
single cell is
transformed with genetic...
-
plasmids containing transgenes are
inserted into the
cells using the
biolistic method or
transkingdom bacterial conjugation (with 10−6 and 10−4 yield...
- the DNA is
often inserted using Agrobacterium-mediated recombination,
biolistics or electroporation. As only a
single cell is
transformed with genetic...
-
agricultural applications.
Genes can be
inserted into
callus cells using biolistic bombardment, also
known as a gene gun, or
Agrobacterium tumefaciens. Cells...
- the cell, namely:
microinjection of
nucleic acid with a fine needle;
biolistic particle delivery, in
which nucleic acid is
attached to
heavy metal particles...
-
chemical sensitisation of cells, electroporation,
optical injection and
biolistics. Finally, the
transfected cells are cultured. As the
aforementioned procedures...
